Products
ZCCHC11 antibody
- Product Name
- ZCCHC11 antibody
- Catalogue No.
- FNab09610
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- zinc finger, CCHC domain containing 11
- Alternative Names
- KIAA0191 antibody, PAPD3 antibody, Terminal uridylyltransferase 4 antibody, TUT4 antibody, TUTase 4 antibody, ZCCHC11 antibody
- UniProt ID
- Q5TAX3
- Observed MW
- 185 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09610(ZCCHC11 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human brain using FNab09610(ZCCHC11 antibody) at dilution of 1:50
- Background
- Uridylyltransferase that mediates the terminal uridylation of mRNAs with short(less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay(PubMed:25480299). Involved in microRNA(miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7(pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem(ES) cell pluripotency(By similarity). Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression(By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.
