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ZC3H12A antibody

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Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Research Area:

Synonyms：	Endoribonuclease ZC3H12A\|Monocyte chemotactic protein-induced protein 1 (MCP-induced protein 1 antibody, MCPIP-1)\|Regnase-1 (Reg1)\|Zinc finger CCCH domain-containing protein 12A\|ZC3H12A\|MCPIP\|MCPIP1 antibody
Catalogue No.：	FNab09601	Reactivity：	Human, Mouse
Host：	Rabbit	Tested Application：	ELISA, WB, IHC, IP
Clonality：	polyclonal	Isotype：	IgG

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
FIGURES
CONDITIONS
FAQS

Product Name: ZC3H12A antibody
Catalogue No.: FNab09601
Size: 100μg
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Clonality: polyclonal
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: zinc finger CCCH-type containing 12A
Alternative Names: Endoribonuclease ZC3H12A|Monocyte chemotactic protein-induced protein 1 (MCP-induced protein 1 antibody, MCPIP-1)|Regnase-1 (Reg1)|Zinc finger CCCH domain-containing protein 12A|ZC3H12A|MCPIP|MCPIP1 antibody
UniProt ID: Q5D1E8
Observed MW: 66 kDa

Application

Tested Applications: ELISA, WB, IHC, IP
Recommended dilution: WB: 1:200-1:1000; IHC: 1:20-1:200; IP: 1:200-1:1000

Validated Images

Raji cells were subjected to SDS PAGE followed by western blot with FNab09601(ZC3H12A Antibody) at dilution of 1:300

IP Result of anti-ZC3H12A (IP:FNab09601, 4ug; Detection:FNab09601 1:300) with Raji cells lysate 2000ug.

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using FNab09601(ZC3H12A Antibody) at dilution of 1:50

Background: Endoribonuclease involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay(PubMed:19909337). Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation(PubMed:26320658). Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines(IL6 and IL2), cell surface receptors(ICOS, TNFRSF4 and TNFR2) and transcription factor(REL)(By similarity). Self regulates by destabilizing its own mRNA(By similarity). Cleaves mRNA harboring a stem-loop(SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner(PubMed:19909337, PubMed:26320658, PubMed:26134560, PubMed:22561375). Plays a role in the inhibition of microRNAs(miRNAs) biogenesis(PubMed:22055188). Cleaves the terminal loop of a set of precursor miRNAs(pre-miRNAs) important for the regulation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs(PubMed:22055188). Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity(PubMed:24048733). Affects the overall ubiquitination of cellular proteins(By similarity). Positively regulates deubiquitinase activity promoting the cleavage at 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors(TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regulating macrophage-mediated inflammatory response and immune homeostasis(By similarity). Induces also deubiquitination of the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regulating the expression of proangiogenic HIF1A-targeted genes(PubMed:24048733). Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimulation or upon DNA damage(PubMed:25861989). Prevents stress granule(SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock and energy deprivation(By similarity). Plays a role in the regulation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state(By similarity). May also act as a transcription factor that regulates the expression of multiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis(PubMed:16574901, PubMed:18364357). Functions as a positive regulator of glial differentiation of neuroprogenitor cells through an amyloid precursor protein(APP)-dependent signaling pathway(PubMed:19185603). Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide(LPS) by reducing I-kappa-B-kinase(IKK)-mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production(By similarity). (Microbial infection) Exhibits broad antiviral activity by cleaving viral RNAs(PubMed:23355615). Binds to Japanese encephalitis virus(JEV) and dengue virus(DEN) RNAs(PubMed:23355615). Exhibits antiviral activity against HIV-1 in lymphocytes by decreasing the abundance of HIV-1 viral RNA species(PubMed:24191027).

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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