Products
WT1 antibody
- Product Name
- WT1 antibody
- Catalogue No.
- FNab09528
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Wilms tumor 1
- Alternative Names
- GUD antibody, WAGR antibody, Wilms tumor 1 antibody, Wilms tumor protein antibody, WIT 2 antibody, WT1 antibody, WT33 antibody
- UniProt ID
- P19544
- Observed MW
- 52 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:5000; IHC: 1:50-1:500; IF: 1:20-1:200; IP: 1:500-1:5000
Validated Images
A549 cells were subjected to SDS PAGE followed by western blot with FNab09528(WT1 antibody) at dilution of 1:1000
IP Result of anti-WT1 (IP:FNab09528, 4ug; Detection:FNab09528 1:1000) with mouse kidney tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using FNab09528 (WT1 antibody) at dilution of 1:200
- Background
- Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
