Products
UPF3A antibody
- Product Name
- UPF3A antibody
- Catalogue No.
- FNab09271
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- UPF3 regulator of nonsense transcripts homolog A(yeast)
- Alternative Names
- HUpf3 antibody, HUPF3A antibody, RENT3A antibody, UPF3 antibody, UPF3A antibody
- UniProt ID
- Q9H1J1
- Observed MW
- 51 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500-1:5000; IHC：1:20-1:200；IF: 1：20-1：200
Validated Images
human testis tissue were subjected to SDS PAGE followed by western blot with FNab09271(UPF3A antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human testis using FNab09271(UPF3A antibody) at dilution of 1:50
- Background
- Involved in nonsense-mediated decay(NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex(EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex(including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.
