HeLa cells were subjected to SDS PAGE followed by western blot with FNab09179(UBE2L3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat heart using FNab09179(UBE2L3 antibody) at dilution of 1:100
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.