mouse thymus tissue were subjected to SDS PAGE followed by western blot with FNab09154(UBE2N antibody) at dilution of 1:1000
IP Result of anti-UBC13 (IP:FNab09154, 4ug; Detection:FNab09154 1:600) with MCF-7 cells lysate 1280ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab09154(UBE2N Antibody) at dilution of 1:200
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair