COLO 320 cells were subjected to SDS PAGE followed by western blot with FNab09137(U2AF35 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab09137(U2AF35 antibody) at dilution of 1:100
Background
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.