Products
THOC5 antibody
- Product Name
- THOC5 antibody
- Catalogue No.
- FNab08668
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- THO complex 5
- Alternative Names
- C22orf19 antibody, KIAA0983 antibody
- UniProt ID
- Q13769
- Observed MW
- 79 kDa
Application
- Tested Applications
- ELISA, IHC, WB, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab08668(THOC5 Antibody) at dilution of 1:600
IP Result of anti-THOC5 (IPFNab08668, 3ug; Detection:FNab08668 1:300) with HEK-293 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08668(THOC5 Antibody) at dilution of 1:50
- Background
- Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex(EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus(KSHV) intronless mRNAs and infectious virus production. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
