Products
RICTOR antibody
- Product Name
- RICTOR antibody
- Catalogue No.
- FNab07297
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- rapamycin-insensitive companion of mTOR
- Alternative Names
- AVO3 homolog antibody, hAVO3 antibody, KIAA1999 antibody, mAVO3 antibody, RICTOR antibody
- UniProt ID
- Q6R327
- Observed MW
- 192 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:1000-1:5000; IHC: 1:50-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab07297(RICTOR antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human lung cancer using FNab07297(RICTOR antibody) at dilution of 1:100
- Background
- Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
