Products
RAD18 antibody
- Product Name
- RAD18 antibody
- Catalogue No.
- FNab07075
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- RAD18 homolog
- Alternative Names
- E3 ubiquitin-protein ligase RAD18|Postreplication repair protein RAD18 (hHR18 antibody, hRAD18)|RING finger protein 73|RING-type E3 ubiquitin transferase RAD18|RAD18|RNF73 antibody
- UniProt ID
- Q9NS91
- Observed MW
- 80 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07075( RAD18 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung using FNab07075(RAD18 antibody) at dilution of 1:50
Immunofluorescence analysis of U2OS cells using FNab07075( RAD18 Antibody) at dilution of 1:100
- Background
- The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
