Products
PKM2-specific antibody
- Product Name
- PKM2-specific antibody
- Catalogue No.
- FNab06495
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- PKM2 pyruvate kinase, muscle
- Alternative Names
- CTHBP antibody, OIP 3 antibody, OIP3 antibody, Opa interacting protein 3 antibody, p58 antibody, PK2 antibody, PK3 antibody, PKM antibody, PKM2 antibody, PK-M2 antibody, PKM2 pyruvate kinase antibody, muscle antibody, Pyruvate kinase 2/3 antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, TCB antibody, THBP1 antibody, Tumor M2 PK antibody
- UniProt ID
- P14618
- Observed MW
- 58 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:1000-1:4000; IP: 1:200-1:1000; IHC: 1:100-1:400; IF: 1:10-1:100
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab06495(PKM2-specific antibody) at dilution of 1:1500
IP Result of anti-PKM2-specific (IP:FNab06495, 4ug; Detection:FNab06495 1:300) with MCF-7 cells lysate 3250ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab06495(PKM2-specific Antibody) at dilution of 1:200
Immunofluorescent analysis of MCF-7 cells, using FNab06495(PKM2-specific Antibody) at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red)
- Background
- PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms .The immunogen of this antibody is M2 isoform, also called PKM2. This antibody is specific to PKM2 isoform.
