Products
NUF2 antibody
- Product Name
- NUF2 antibody
- Catalogue No.
- FNab05909
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- NUF2, NDC80 kinetochore complex component, homolog
- Alternative Names
- Kinetochore protein Nuf2 (hNuf2 antibody, hNuf2R antibody, hsNuf2)|Cell division cycle-associated protein 1|NUF2|CDCA1|NUF2R antibody
- UniProt ID
- Q9BZD4
- Observed MW
- 50 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab05909(NUF2 antibody) at dilution of 1:500
IP Result of anti-NUF2 (IP:FNab05909, 3ug; Detection:FNab05909 1:500) with HEK-293 cells lysate 1760ug.
- Background
- Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity(PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore(PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules(PubMed:23085020).
