Products
MMACHC antibody
- Product Name
- MMACHC antibody
- Catalogue No.
- FNab05227
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- methylmalonic aciduria(cobalamin deficiency) cblC type, with homocystinuria
- UniProt ID
- Q9Y4U1
- Observed MW
- 32 kDa
Application
- Tested Applications
- ELISA, WB
- Recommended dilution
- WB: 1:500-1:2000
Validated Images
Raji cells were subjected to SDS PAGE followed by western blot with FNab05227( MMACHC Antibody) at dilution of 1:600
- Background
- Catalyzes the reductive dealkylation of cyanocobalamin to cob(II)alamin, using FAD or FMN as cofactor and NADPH as cosubstrate(PubMed:19700356, PubMed:21697092, PubMed:22642810). Can also catalyze the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin(PubMed:19801555, PubMed:22642810, PubMed:25809485). Under anaerobic conditions cob(I)alamin is the first product; it is highly reactive and is converted to aquocob(II)alamin in the presence of oxygen(PubMed:19801555). Binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives(PubMed:21071249).
