Products

anti- MGEA5 antibody

FNab05163 Catalogue No.
Synonyms
HEXC, KIAA0679, MEA5
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, WB, IHC, IF, IP
Product Information
Product Name
anti- MGEA5 antibody
Catalogue No.
FNab05163
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.
Immunogen Information
Immunogen
meningioma expressed antigen 5(hyaluronidase)
Synonyms
HEXC, KIAA0679, MEA5
Observed MW
130 kDa
Application
Tested Application
ELISA, WB, IHC, IF, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Uniprot information
UniProt ID
O60502
Images
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using FNab05163(MGEA5 Antibody) at dilution of 1:200
IP Result of anti-MGEA5 (IP:FNab05163, 3ug; Detection:FNab05163 1:500) with SH-SY5Y cells lysate 2900ug.
BxPC-3 cells were subjected to SDS PAGE followed by western blot with FNab05163(MGEA5 Antibody) at dilution of 1:1500