Products
HIF1a antibody
Category:
Research Area:
- SPECIFICATIONS
- Product Name
- HIF1a antibody
- Catalogue No.
- FNab03859
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
- Alternative Names
- BHLHE78 antibody, MOP1 antibody, PASD8 antibody
- UniProt ID
- Q16665
- Observed MW
- 120 kDa
Application
- Tested Applications
- ELISA, IHC, IF, FC, WB
- Recommended dilution
- WB: 1:200-1:1000; IHC: 1:50-1:200; IF: 1:10-1:100
Validated Images
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with FNab03859(HIF1a Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human thyroid cancer using FNab03859(HIF1A antibody) at dilution of 1:100
Immunofluorescent analysis of Hela cells, using HIF1A antibody FNab03859 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.