Products
HERC5 antibody
- Product Name
- HERC5 antibody
- Catalogue No.
- FNab03837
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- hect domain and RLD 5
- Alternative Names
- CEB1 antibody, CEBP1 antibody
- UniProt ID
- Q9UII4
- Observed MW
- 128 kDa
Application
- Tested Applications
- ELISA, WB
- Recommended dilution
- WB: 1:500-1:2000
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab03837(HERC5 antibody) at dilution of 1:500
- Background
- Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
