Products
ERp72 antibody
- Product Name
- ERp72 antibody
- Catalogue No.
- FNab02859
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- protein disulfide isomerase family A, member 4
- Alternative Names
- ER protein 70 antibody, ER protein 72 antibody, ERp 72 antibody, ERP70 antibody, ERP72 antibody, PDIA4 antibody, Protein disulfide isomerase A4 antibody
- UniProt ID
- P13667
- Observed MW
- 72 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
Various lysates were subjected to SDS PAGE followed by western blot with FNab02859(PDIA4 antibody) at dilution of 1:500
IP Result of anti-ERp72 (IP: FNab02859, 4ug; Detection: FNab02859 1:500) with HeLa cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human lung cancer using FNab02859(PDIA4 antibody) at dilution of 1:100
Immunofluorescent analysis of Hela cells, using ERp72 antibody FNab02859 at 1:50 dilution and FITC-labeled goat anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Background
- PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of-S-S-bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357).
