Products
ERCC1 antibody
- Product Name
- ERCC1 antibody
- Catalogue No.
- FNab02832
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- excision repair cross-complementing rodent repair deficiency, complementation group 1(includes overlapping antisense sequence)
- UniProt ID
- P07992
- Observed MW
- 36-38 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab02832(ERCC1 antibody) at dilution of 1:300
IP Result of anti-ERCC1 (IP:FNab02832, 3ug; Detection:FNab02832 1:300) with MCF-7 cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human testis using FNab02832(ERCC1 antibody) at dilution of 1:50
- Background
- Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links(ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair(HDR) of DNA double-strand breaks, in conjunction with SLX4.
