HeLa cells were subjected to SDS PAGE followed by western blot with FNab02619(ECD antibody) at dilution of 1:300
IP Result of anti-ECD (IP:FNab02619, 4ug; Detection:FNab02619 1:300) with HeLa cells lysate 2480ug.
Background
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP(PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes(PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins(PubMed:19640839). The cell cycle-regulating function may depend on its RUVBL1-mediated association with the R2TP complex(PubMed:26711270). May play a role in regulation of pre-mRNA splicing(PubMed:24722212).