anti- DHFR antibody
FNab02365
Catalogue No.
- Synonyms
- DHFR, DHFRP1, dihydrofolate reductase
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Isotype
- IgG
- Tested Application
- ELISA, WB, IHC, IP, IF
Product Information
- Product Name
- anti- DHFR antibody
- Catalogue No.
- FNab02365
- Size
- 100µg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Host
- Rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
- Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Immunogen Information
- Immunogen
- dihydrofolate reductase
- Synonyms
- DHFR, DHFRP1, dihydrofolate reductase
- Observed MW
- 21 kDa
Application
- Tested Application
- ELISA, WB, IHC, IP, IF
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF： 1:20-1:200
Uniprot information
- UniProt ID
- P00374
Images
- Image 1
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Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab02365(DHFR Antibody) at dilution of 1:50
- Image 2
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Immunofluorescent analysis of HeLa cells using FNab02365 (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Image 3
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IP Result of anti-DHFR (IP: FNab02365, 3ug; Detection: FNab02365 1:500) with Raji cells lysate 2000ug
- Image 4
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K-562 cells were subjected to SDS PAGE followed by western blot with FNab02365(DHFR antibody) at dilution of 1:500