Products

anti- DHFR antibody

FNab02365 Catalogue No.
Synonyms:
DHFR, DHFRP1, dihydrofolate reductase
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Isotype:
IgG
Tested Application:
ELISA, WB, IHC, IP, IF
Product Information
Product Name
anti- DHFR antibody
Catalogue No.
FNab02365
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Immunogen Information
Immunogen
dihydrofolate reductase
Synonyms
DHFR, DHFRP1, dihydrofolate reductase
Observed MW
21 kDa
Application
Tested Application
ELISA, WB, IHC, IP, IF
Recommended dilution
WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Uniprot information
UniProt ID
P00374
Images
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab02365(DHFR Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using FNab02365 (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
IP Result of anti-DHFR (IP: FNab02365, 3ug; Detection: FNab02365 1:500) with Raji cells lysate 2000ug
K-562 cells were subjected to SDS PAGE followed by western blot with FNab02365(DHFR antibody) at dilution of 1:500