Products
DGCR8 C-Terminal antibody
- Product Name
- DGCR8 C-Terminal antibody
- Catalogue No.
- FNab02351
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- DiGeorge syndrome critical region gene 8
- Alternative Names
- C22orf12 antibody, DGCR8 antibody, DGCRK6 antibody, Gy1 antibody
- UniProt ID
- Q8WYQ5
- Observed MW
- 120 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP, FC
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab02351(DGCR8 antibody) at dilution of 1:1000
IP Result of anti-DGCR8 C-terminal (IP:FNab02351, 3ug; Detection:FNab02351 1:800) with HEK-293 cells lysate 2700ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab02351(DGCR8 antibody) at dilution of 1:100
- Background
- Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA(miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts(pri-miRNAs) to release precursor miRNA(pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs(PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer(of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding(PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine(m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing(PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal(By similarity).
