Products
KRT8 antibody
- Product Name
- KRT8 antibody
- Catalogue No.
- FNab02221
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- keratin 8
- Alternative Names
- Keratin antibody, type II cytoskeletal 8|Cytokeratin-8 (CK-8)|Keratin-8 (K8)|Type-II keratin Kb8|KRT8|CYK8 antibody
- UniProt ID
- P05787
- Observed MW
- 53 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab02221(KRT8 antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human colon tissue slide using FNab02221( KRT8 Antibody) at dilution of 1:200
Immunofluorescent analysis of HepG2 cells using FNab02221 ( KRT8 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
- Background
- This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
