Jurkat cells were subjected to SDS PAGE followed by western blot with FNab01458(CD3E antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using FNab01458( CD3E Antibody) at dilution of 1:200 Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.