Products
CD146/MCAM antibody
- Product Name
- CD146/MCAM antibody
- Catalogue No.
- FNab01429
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 0E4
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- melanoma cell adhesion molecule
- Alternative Names
- CD146 antibody, MCAM antibody, MUC18 antibody, Cell surface glycoprotein MUC18 antibody, Cell surface glycoprotein P1H12 antibody, Melanoma cell adhesion molecule antibody, Melanoma-associated antigen A32 antibody, Melanoma-associated antigen MUC18 antibody
- UniProt ID
- P43121
- Observed MW
- 113-120 kDa
Application
- Tested Applications
- ELISA, WB, IHC, FC
- Recommended dilution
- WB: 1:500-1:5000; IHC: 1:20-1:200
Validated Images
A375 cells were subjected to SDS PAGE followed by western blot with FNab01429(CD146, MCAM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab01429(CD146, MCAM Antibody) at dilution of 1:20
- Background
- CD146, also known as melanoma cell adhesion molecule(MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin(Ig) superfamily(PMID: 8378324). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells(CECs)(PMID: 19356677).
