CAPZA1 antibody

Catalogue No.:FNab01255Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
CAPZA1 antibody
Catalogue No.
FNab01255
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
capping protein(actin filament) muscle Z-line, alpha 1
UniProt ID
P52907
Observed MW
33kd
Application
Tested Applications
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:200
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab01255(CAPZA1 antibody) at dilution of 1:400
IP Result of anti-CAPZA1 (IP:FNab01255, 4ug; Detection:FNab01255 1:300) with SH-SY5Y cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human ovary tumor using FNab01255(CAPZA1 antibody) at dilution of 1:50
Background
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments(barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins(such as gelsolin and severin), these proteins do not sever actin filaments.