HeLa cells were subjected to SDS PAGE followed by western blot with FNab00975(BTD antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer using FNab00975(BTD antibody) at dilution of 1:100
Background
The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described.