Products
ATG4B antibody
- Product Name
- ATG4B antibody
- Catalogue No.
- FNab00674
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ATG4 autophagy related 4 homolog B
- Alternative Names
- APG4B antibody, AUTL1 antibody, KIAA0943 antibody
- UniProt ID
- Q9Y4P1
- Observed MW
- 44 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000 ; IHC: 1:20-1:200
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab00674(ATG4B antibody) at dilution of 1:800
IP Result of anti-ATG4B (IP:FNab00674, 4ug; Detection:FNab00674 1:500) with mouse skeletal muscle tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab00674(ATG4B antibody) at dilution of 1:50
- Background
- Cysteine protease required for the cytoplasm to vacuole transport(Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine(PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
