HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00558(ARHGEF1 antibody) at dilution of 1:1000
IP Result of anti-ARHGEF1 (IP:FNab00558, 4ug; Detection:FNab00558 1:1000) with HEK-293 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human colon using FNab00558(ARHGEF1 antibody) at dilution of 1:50
Background
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12(GNA12) and alpha-13(GNA13) subunits. Acts as GTPase-activating protein(GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor(GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.