Products
ANAPC5 antibody
- Product Name
- ANAPC5 antibody
- Catalogue No.
- FNab00387
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- anaphase promoting complex subunit 5
- Alternative Names
- Anaphase-promoting complex subunit 5 (APC5)|Cyclosome subunit 5|ANAPC5|APC5 antibody
- UniProt ID
- Q9UJX4
- Observed MW
- 85 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Validated Images
BxPC-3 cells were subjected to SDS PAGE followed by western blot with FNab00387(ANAPC5 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat kidney using FNab00387(ANAPC5 antibody) at dilution of 1:50
- Background
- This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
