ANAPC5 antibody

Synonyms:Anaphase-promoting complex subunit 5 (APC5)|Cyclosome subunit 5|ANAPC5|APC5 antibody
Catalogue No.:FNab00387Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
  • FIGURES
  • CONDITIONS
Product Name
ANAPC5 antibody
Catalogue No.
FNab00387
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
anaphase promoting complex subunit 5
Alternative Names
Anaphase-promoting complex subunit 5 (APC5)|Cyclosome subunit 5|ANAPC5|APC5 antibody
UniProt ID
Q9UJX4
Observed MW
85 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Validated Images
BxPC-3 cells were subjected to SDS PAGE followed by western blot with FNab00387(ANAPC5 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat kidney using FNab00387(ANAPC5 antibody) at dilution of 1:50
Background
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.