Products
HRP-SA(Streptavidin Horseradish Peroxidase)Activity ELISA Kit
- Product Name
- HRP-SA(Streptavidin Horseradish Peroxidase)Activity ELISA Kit
- Alias
- HRP-SA ELISA Kit, Streptavidin Horseradish Peroxidase ELISA Kit
- Catalogue No.
- EU2619
- Size
- 48T/96T
- Species
- Universal
- Sample Type
- Quantitative?detection?of?HRP-SA?(horseradish?peroxidase?conjugated?streptavidin)
- Detection Method
- Direct ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 1 hours
- Range
- 28.125-1800uIU/ml(1800uU=6ng)
- Sensitivity
- 14uIU/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with SA-HRP , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(uIU/ml) OD-1 OD-2 Average 0 0.153 0.157 0.155 28.125 0.191 0.197 0.194 56.25 0.281 0.289 0.285 112.5 0.419 0.431 0.425 225 0.597 0.615 0.606 450 0.909 0.935 0.922 900 1.606 1.652 1.629 1800 2.21 2.274 2.242 - Recovery
-
Add a certain amount of SA-HRP into the sample. Calculate the recovery by comparing the measured value with the expected amount of SA-HRP in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 88-101 96 EDTA plasma(n=10) 92-104 98 Heparin plasma(n=10) 87-99 95 - Linearity
-
Dilute the sample with a certain amount of SA-HRP at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 87-105% 87-97% 85-100% EDTA plasma(n=10) 93-100% 83-98% 85-98% Heparin plasma(n=10) 88-98% 82-94% 82-96% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (uIU/ml) 57.23 222 892 56.58 226 878 Standard deviation 2.32 10.41 55.57 2.95 12.93 50.22 CV(%) 4.06 4.69 6.23 5.22 5.72 5.72 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
