POD(peroxidase) Assay kit

Category:
Catalogue No.: 
EU2611
Alias: 
POD ELISA Kit, peroxidase ELISA Kit
Species: 
Universal
Range: 
3.12-200pmol/ml
Sensitivity: 
1.875pg/ml
Manual MSDS
  • SPECIFICATIONS
Product Name
POD(peroxidase) Assay kit
Alias
POD ELISA Kit, peroxidase ELISA Kit
Catalogue No.
EU2611
Size
48T/96T
Species
Universal
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Enzyme catalysis
Detection Wavelength
OD450
Reaction Duration
20 minutes
Range
3.12-200pmol/ml
Sensitivity
1.875pg/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with POD , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pmol/ml) OD-1 OD-2 Average
0 0.07 0.066 0.068
3.125 0.275 0.23 0.252
6.25 0.333 0.344 0.338
12.5 0.582 0.609 0.595
25 0.817 0.82 0.818
50 1.412 1.435 1.423
100 1.81 1.892 1.851
200 2.692 2.615 2.653
EU2611 Standard Curve Image
Recovery

Add a certain amount of POD into the sample. Calculate the recovery by comparing the measured value with the expected amount of POD in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 91-104 99
EDTA plasma(n=10) 90-104 96
Heparin plasma(n=10) 95-105 99
Linearity

Dilute the sample with a certain amount of POD at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 90-98% 88-100% 85-100%
EDTA plasma(n=10) 84-99% 83-99% 82-96%
Heparin plasma(n=10) 83-93% 80-93% 86-100%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pmol/ml) 5.88 23.69 102.4 6 23.85 101.41
Standard deviation 0.31 1.21 5.56 0.28 1.49 5.05
CV(%) 5.32 5.12 5.43 4.69 6.23 4.98
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
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