Products
Rabbit OVA sIgE (Ovalbumin Specific IgE) ELISA Kit
- Product Name
- Rabbit OVA sIgE (Ovalbumin Specific IgE) ELISA Kit
- Alias
- OVA sIgE ELISA Kit, Ovalbumin Specific IgE ELISA Kit
- Catalogue No.
- ERB0234
- Size
- 48T/96T
- Species
- Rabbit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Capture ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 3 hours
- Range
- 1.563-100ng/ml
- Sensitivity
- 0.938ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with OVA sIgE , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 HRP-Antigen(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antigen Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) OD-1 OD-2 Average 0 0.08 0.082 0.081 1.562 0.174 0.18 0.177 3.125 0.251 0.259 0.255 6.25 0.451 0.465 0.458 12.5 0.724 0.746 0.735 25 1.203 1.237 1.22 50 1.637 1.685 1.661 100 2.093 2.153 2.123 
- Recovery
-
Add a certain amount of OVA sIgE into the sample. Calculate the recovery by comparing the measured value with the expected amount of OVA sIgE in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 94-103 99 EDTA plasma(n=10) 85-105 96 Heparin plasma(n=10) 85-101 94 - Linearity
-
Dilute the sample with a certain amount of OVA sIgE at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 85-104% 86-99% 86-96% EDTA plasma(n=10) 90-101% 86-101% 85-100% Heparin plasma(n=10) 88-100% 85-100% 87-99% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 3.17 11.24 48.97 3.11 12.64 50.28 Standard deviation 0.15 0.56 2.39 0.16 0.69 2.64 CV(%) 4.69 4.96 4.89 5.27 5.42 5.26 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
