Products

Rat SP(Substance P) ELISA Kit

ER1880 Catalogue No.
SP ELISA Kit
Reactivity
Rat
Range
7.813-500pg/ml
Sensitivity
4.688pg/ml
Product Name
Rat SP(Substance P) ELISA Kit
Catalogue No.
ER1880
Alias
SP ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
 SP ELISA Kit allows for the in vitro quantitative determination of SP concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Rat
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of SP and the recovery rates were calculated by comparing the measured value to the expected amount of SP in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)89-9793
EDTA plasma(n=5)90-9794
heparin plasma(n=5)87-10494
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)93-101%86-104%87-103%96-103%
EDTA plasma(n=5)83-99%86-101%86-99%85-101%
heparin plasma(n=5)89-97%80-97%81-94%82-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only