- Product Name
- Rat SP(Substance P) ELISA Kit
- Catalogue No.
- SP ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- SP ELISA Kit allows for the in vitro quantitative determination of SP concentrations in plasma, tissue homogenates and other biological fluids.
- < 4.688pg/ml
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of SP and the recovery rates were calculated by comparing the measured value to the expected amount of SP in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 89-97 93 EDTA plasma(n=5) 90-97 94 heparin plasma(n=5) 87-104 94
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 93-101% 86-104% 87-103% 96-103% EDTA plasma(n=5) 83-99% 86-101% 86-99% 85-101% heparin plasma(n=5) 89-97% 80-97% 81-94% 82-96%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area