Products

Rat PYGL(Glycogen phosphorylase, liver form) ELISA Kit

ER0386 Catalogue No.
Reactivity
Rat
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Rat PYGL(Glycogen phosphorylase, liver form) ELISA Kit
Catalogue No.
ER0386
Detection Method
Sandwich ELISA, Double Antibody
Application
PYGL ELISA Kit allows for the in vitro quantitative determination of PYGL concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
P09811
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of PYGL and the recovery rates were calculated by comparing the measured value to the expected amount of PYGL in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10597
EDTA plasma(n=5)88-9391
heparin plasma(n=5)92-10398
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYGL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)92-102%86-104%95-104%85-103%
EDTA plasma(n=5)84-96%83-100%83-99%85-100%
heparin plasma(n=5)82-100%85-97%83-99%81-88%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only