Products
Monkey NSE(Neuron-specific Enolase) ELISA Kit
- Product Name
- Monkey NSE(Neuron-specific Enolase) ELISA Kit
- Alias
- NSE ELISA Kit, ENO2 ELISA Kit, gamma-Enolase ELISA Kit, enolase 2(gamma, neuronal) ELISA Kit, gamma-enolase ELISA Kit, Neural enolase ELISA Kit, neuron specific gamma enolase ELISA Kit, Neuron-specific enolase ELISA Kit
- Catalogue No.
- EMK0214
- Size
- 48T/96T
- Species
- Monkey
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 0.313-20ng/ml
- Sensitivity
- 0.188ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with NSE , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) OD-1 OD-2 Average 0 0.112 0.116 0.114 0.312 0.152 0.156 0.154 0.625 0.196 0.202 0.199 1.25 0.293 0.301 0.297 2.5 0.526 0.542 0.534 5 0.865 0.891 0.878 10 1.507 1.551 1.529 20 2.163 2.225 2.194 
- Recovery
-
Add a certain amount of NSE into the sample. Calculate the recovery by comparing the measured value with the expected amount of NSE in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 86-102 93 EDTA plasma(n=10) 88-96 92 Heparin plasma(n=10) 88-102 94 - Linearity
-
Dilute the sample with a certain amount of NSE at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 87-99% 85-95% 85-100% EDTA plasma(n=10) 88-97% 94-99% 89-96% Heparin plasma(n=10) 89-101% 85-93% 86-100% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 0.59 2.54 9.53 0.61 2.55 9.84 Standard deviation 0.03 0.11 0.51 0.04 0.14 0.56 CV(%) 5.34 4.23 5.39 5.85 5.42 5.72 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
