- Product Name
- Mouse Pycard(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit
- Catalogue No.
- EM8060
- Alias
- ASC ELISA Kit, CARD5 ELISA Kit, hASC ELISA Kit, PYCARD ELISA Kit, PYD and CARD domain containing ELISA Kit, TMS ELISA Kit, TMS 1 ELISA Kit, TMS1 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- Pycard ELISA Kit allows for the in vitro quantitative determination of Pycard concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.25-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Mouse
- UniProt ID
- Q9EPB4
- Standard curve

- Storage
- 2-8 °C for 6 months
- Recovery
Matrices listed below were spiked with certain level of Pycard and the recovery rates were calculated by comparing the measured value to the expected amount of Pycard in samples.
Matrix | Recovery range(%) | Average(%) |
serum(n=5) | 93-102 | 97 |
EDTA plasma(n=5) | 85-102 | 91 |
heparin plasma(n=5) | 85-100 | 91 |
- Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pycard and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 |
serum(n=5) | 85-100% | 85-103% | 86-104% |
EDTA plasma(n=5) | 86-99% | 82-101% | 83-101% |
heparin plasma(n=5) | 80-98% | 88-100% | 80-96% |
- CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10%
- Note
- For Research Use Only