Products

Mouse Pycard(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit

EM8060 Catalogue No.
ASC ELISA Kit, CARD5 ELISA Kit, hASC ELISA Kit, PYCARD ELISA Kit, PYD and CARD domain containing ELISA Kit, TMS ELISA Kit, TMS 1 ELISA Kit, TMS1 ELISA Kit
Reactivity
Mouse
Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Product Name
Mouse Pycard(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit
Catalogue No.
EM8060
Alias
ASC ELISA Kit, CARD5 ELISA Kit, hASC ELISA Kit, PYCARD ELISA Kit, PYD and CARD domain containing ELISA Kit, TMS ELISA Kit, TMS 1 ELISA Kit, TMS1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Pycard ELISA Kit allows for the in vitro quantitative determination of Pycard concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
Q9EPB4
Standard curve
Storage
2-8 °C for 6 months
Recovery

Matrices listed below were spiked with certain level of Pycard and the recovery rates were calculated by comparing the measured value to the expected amount of Pycard in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)93-10297
EDTA plasma(n=5)85-10291
heparin plasma(n=5)85-10091
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pycard and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)85-100%85-103%86-104%
EDTA plasma(n=5)86-99%82-101%83-101%
heparin plasma(n=5)80-98%88-100%80-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only