Products
Mouse Pah (Phenylalanine-4-hydroxylase) ELISA Kit
- Product Name
- Mouse Pah (Phenylalanine-4-hydroxylase) ELISA Kit
- Alias
- Pah ELISA Kit, Phenylalanine-4-hydroxylase ELISA Kit, Phe-4-monooxygenase ELISA Kit
- Catalogue No.
- EM7886
- Size
- 48T/96T
- Species
- Mouse
- UniProt No.
- P16331
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 31.25-2000pg/ml
- Sensitivity
- 18.75pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with Pah , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.085 0.087 0.086 31.25 0.134 0.138 0.136 62.5 0.264 0.272 0.268 125 0.384 0.396 0.39 250 0.587 0.605 0.596 500 0.806 0.83 0.818 1000 1.355 1.395 1.375 2000 2.137 2.199 2.168 
- Recovery
-
Add a certain amount of Pah into the sample. Calculate the recovery by comparing the measured value with the expected amount of Pah in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 85-103 93 EDTA plasma(n=10) 89-102 97 Heparin plasma(n=10) 87-102 94 - Linearity
-
Dilute the sample with a certain amount of Pah at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 89-101% 87-101% 89-97% EDTA plasma(n=10) 86-99% 85-100% 85-100% Heparin plasma(n=10) 87-104% 87-100% 92-100% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 62.15 241.8 1002.3 60.01 257.6 1010.4 Standard deviation 2.85 9.91 45.81 2.44 12.06 41.22 CV(%) 4.59 4.1 4.57 4.06 4.68 4.08 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
