Products
Mouse Hadh (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) ELISA Kit
- Catalogue No.:
- EM7221
- Alias:
- Hadh ELISA Kit, Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial ELISA Kit, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase ELISA Kit, Short-chain 3-hydroxyacyl-CoA dehydrogenase ELISA Kit, HCDH ELISA Kit
- Species:
- Mouse
- Range:
- 78-5000pg/ml
- Sensitivity:
- 46.9pg/ml
- SPECIFICATIONS
- Product Name
- Mouse Hadh (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) ELISA Kit
- Alias
- Hadh ELISA Kit, Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial ELISA Kit, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase ELISA Kit, Short-chain 3-hydroxyacyl-CoA dehydrogenase ELISA Kit, HCDH ELISA Kit
- Catalogue No.
- EM7221
- Size
- 48T/96T
- Species
- Mouse
- UniProt No.
- Q61425
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 78-5000pg/ml
- Sensitivity
- 46.9pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with Hadh , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.1 0.102 0.101 78.125 0.181 0.187 0.184 156.25 0.23 0.236 0.233 312.5 0.399 0.411 0.405 625 0.662 0.682 0.672 1250 1.065 1.095 1.08 2500 1.535 1.579 1.557 5000 2.054 2.114 2.084 - Recovery
-
Add a certain amount of Hadh into the sample. Calculate the recovery by comparing the measured value with the expected amount of Hadh in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 86-98 91 EDTA plasma(n=10) 94-101 97 Heparin plasma(n=10) 87-100 92 - Linearity
-
Dilute the sample with a certain amount of Hadh at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 88-100% 85-101% 85-99% EDTA plasma(n=10) 86-105% 85-96% 87-99% Heparin plasma(n=10) 92-103% 87-101% 85-99% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 157.2 609.6 2426.3 160.7 628 2520 Standard deviation 8.08 30.05 122.29 7.84 29.7 128.52 CV(%) 5.14 4.93 5.04 4.88 4.73 5.1 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100