Products
Mouse NFkB(Nuclear Factor Kappa B) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NFkB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NFkB bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NFkB in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EM1230
- Alias:
- NFKB ELISA Kit, Nuclear factor NF-kappa-B ELISA Kit, Nuclear factor NF-kappa-B p100 subunit ELISA Kit, DNA-binding factor KBF2 ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 ELISA Kit, Nfkb2 ELISA Kit, Nuclear factor NF-kappa-B p105 subunit ELISA Kit, DNA-binding factor KBF1 ELISA Kit, EBP-1 ELISA Kit, NF-kappa-B1 p84/NF-kappa-B1 p98 ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit, Nfkb1 ELISA Kit
- Species:
- Mouse
- Range:
- 15.625-1000pg/ml
- Sensitivity:
- 9.375pg/ml
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- Product Name
- Mouse NFkB(Nuclear Factor Kappa B) ELISA Kit
- Alias
- NFKB ELISA Kit, Nuclear factor NF-kappa-B ELISA Kit, Nuclear factor NF-kappa-B p100 subunit ELISA Kit, DNA-binding factor KBF2 ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 ELISA Kit, Nfkb2 ELISA Kit, Nuclear factor NF-kappa-B p105 subunit ELISA Kit, DNA-binding factor KBF1 ELISA Kit, EBP-1 ELISA Kit, NF-kappa-B1 p84/NF-kappa-B1 p98 ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit, Nfkb1 ELISA Kit
- Catalogue No.
- EM1230
- Size
- 48T/96T
- Species
- Mouse
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 15.625-1000pg/ml
- Sensitivity
- 9.375pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with NFkB , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
- Washing: Wash the plate twice without immersing.
- Step 2: Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37°C.
- Washing: Wash the plate three times and immerse for 1min each time.
- Step 3: Add 100ul HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37°C.
- Washing: Wash the plate five times and immerse for 1min each time.
- Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
- Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.103 0.105 0.104 15.625 0.201 0.207 0.204 31.25 0.285 0.293 0.289 62.5 0.426 0.438 0.432 125 0.657 0.677 0.667 250 1.019 1.049 1.034 500 1.488 1.532 1.51 1000 2.063 2.123 2.093 - Recovery
-
Add a certain amount of NFkB into the sample. Calculate the recovery by comparing the measured value with the expected amount of NFkB in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 85-98 95 EDTA plasma(n=10) 87-101 92 Heparin plasma(n=10) 88-101 92 - Linearity
-
Dilute the sample with a certain amount of NFkB at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 82-97% 85-99% 88-100% EDTA plasma(n=10) 83-94% 80-99% 84-100% Heparin plasma(n=10) 86-99% 82-96% 83-100% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 30.42 119.2 499 31.45 125.6 503 Standard deviation 1.79 6.94 31.54 1.64 7.84 31.79 CV(%) 5.88 5.82 6.32 5.23 6.24 6.32 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
- Journal:
- International Journal of Biological Macromolecules
- Author:
- College of Chemical Enginering, Huaqiao University, Xiamen 362021, China.
- Cited Date:
- 2024-09-20
- Product:
- Journal:
- Biomedicine & Pharmacotherapy
- Author:
- Pharmacology and Toxicology Department, Faculty of Pharmacy, Damanhour University, 22511,?Egypt; Faculty of Health Sciences Technology, Borg Al Arab Technological University, New Borg El Arab, Egypt.
- Sample:
- serum
- Cited Date:
- 2024-10-08
- Product:
- Journal:
- International Journal of Pharmaceutics
- Author:
- Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt
- Cited Date:
- 2023-12-01
- Product:
- Journal:
- International Journal of Pharmaceutics
- Author:
- Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt
- Cited Date:
- 2023-09-22
- Product:
- Journal:
- International Immunopharmacology
- Cited Date:
- 2023-02-17
- Product:
- Journal:
- Drug Delivery and Translational Research
- Author:
- Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
- Cited Date:
- 2023-09-08
- Product:
- Journal:
- International Immunopharmacology
- Author:
- Department of Pharmacognosy, Faculty of Pharmacy, Helwan University., Cairo 11795, Egypt.
- Cited Date:
- 2024-08-02
- Product:
- Journal:
- Neurotoxicity Research
- Cited Date:
- 2019-11-05
- Product:
- Journal:
- Toxicology and Applied Pharmacology
- Author:
- Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
- Cited Date:
- 2024-09-27
- Product: