Products
Dog Anti-KLH IgM (anti Keyhole Limpet Hemocyanin IgM) ELISA Kit
- Product Name
- Dog Anti-KLH IgM (anti Keyhole Limpet Hemocyanin IgM) ELISA Kit
- Alias
- Anti-KLH IgM ELISA Kit, anti Keyhole Limpet Hemocyanin IgM ELISA Kit
- Catalogue No.
- ECA0112
- Size
- 48T/96T
- Species
- Dog
- UniProt No.
- 9013-72-3
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Capture ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 0.313-20ng/ml
- Sensitivity
- 0.188ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with Anti-KLH IgM , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antigen(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antigen Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) OD-1 OD-2 Average 0 0.111 0.116 0.113 0.312 0.158 0.166 0.161 0.625 0.228 0.24 0.233 1.25 0.302 0.317 0.308 2.5 0.527 0.554 0.538 5 0.876 0.921 0.894 10 1.457 1.532 1.487 20 2.137 2.246 2.181 
- Recovery
-
Add a certain amount of Anti-KLH IgM into the sample. Calculate the recovery by comparing the measured value with the expected amount of Anti-KLH IgM in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 85-100 93 EDTA plasma(n=10) 85-101 94 Heparin plasma(n=10) 85-100 94 - Linearity
-
Dilute the sample with a certain amount of Anti-KLH IgM at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 94-102% 93-101% 87-96% EDTA plasma(n=10) 92-102% 95-101% 83-95% Heparin plasma(n=10) 86-104% 91-99% 80-97% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 0.65 2.53 10.21 0.6 2.38 10.04 Standard deviation 0.04 0.16 0.53 0.03 0.12 0.59 CV(%) 5.43 6.42 5.23 5.02 5.08 5.85 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
