- Product Name
- Bovine IL12A(Interleukin-12 subunit alpha) ELISA KIT
- Catalogue No.
- Detection Method
- Sandwich ELISA, Double Antibody
- IL12A ELISA KIT allows for the in vitro quantitative determination of IL12A concentrations in plasma, tissue homogenates and other biological fluids.
- < 9.375pg/ml
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of IL12A and the recovery rates were calculated by comparing the measured value to the expected amount of IL12A in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 97-104 100 EDTA plasma(n=5) 85-99 92 heparin plasma(n=5) 85-105 95
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL12A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 87-103% 85-98% 85-97% 90-102% EDTA plasma(n=5) 82-98% 86-99% 87-97% 82-98% heparin plasma(n=5) 81-98% 81-93% 83-97% 87-98%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area