Products

Bovine IL-12A(Interleukin-12 subunit alpha) ELISA Kit

EB0170 Catalogue No.
IL12A ELISA Kit
Reactivity
Bovine
Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Product Name
Bovine IL-12A(Interleukin-12 subunit alpha) ELISA Kit
Catalogue No.
EB0170
Alias
IL12A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-12A ELISA Kit allows for the in vitro quantitative determination of IL12A concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Bovine
UniProt ID
P54349
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL12A and the recovery rates were calculated by comparing the measured value to the expected amount of IL12A in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-9994
EDTA plasma(n=5)86-10394
heparin plasma(n=5)90-9692
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL12A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-105%96-102%85-103%90-103%
EDTA plasma(n=5)82-97%89-101%83-101%84-99%
heparin plasma(n=5)84-94%83-96%80-96%82-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only