Bovine LIF(Leukemia inhibitory factor) ELISA Kit
- Product Name
- Bovine LIF(Leukemia inhibitory factor) ELISA Kit
- Catalogue No.
- EB0006
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- LIF ELISA Kit allows for the in vitro quantitative determination of LIF concentrations in plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 31.2-2000pg/ml
- Sensitivity
- < 18.75pg/ml
- Species
- Bovine
- UniProt ID
- Q27956
- Standard curve
- Storage
- 4 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of LIF and the recovery rates were calculated by comparing the measured value to the expected amount of LIF in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 90-105 100 EDTA plasma(n=5) 86-105 93 heparin plasma(n=5) 86-98 90 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 96-104% 88-105% 89-97% 85-105% EDTA plasma(n=5) 82-94% 86-101% 83-98% 84-101% heparin plasma(n=5) 81-98% 80-87% 81-94% 85-98% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only