Products

Bovine LIF(Leukemia inhibitory factor) ELISA Kit

EB0006 Catalogue No.
Reactivity
Bovine
Range
31.2-2000pg/ml
Sensitivity
18.75pg/ml
Product Name
Bovine LIF(Leukemia inhibitory factor) ELISA Kit
Catalogue No.
EB0006
Detection Method
Sandwich ELISA, Double Antibody
Application
LIF ELISA Kit allows for the in vitro quantitative determination of LIF concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.2-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Bovine
UniProt ID
Q27956
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of LIF and the recovery rates were calculated by comparing the measured value to the expected amount of LIF in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)90-105100
EDTA plasma(n=5)86-10593
heparin plasma(n=5)86-9890
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)96-104%88-105%89-97%85-105%
EDTA plasma(n=5)82-94%86-101%83-98%84-101%
heparin plasma(n=5)81-98%80-87%81-94%85-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only