COVID-19 Spike Protein(RBD) Accquant ELISA Kit

Category: Research Area:
Catalogue No.: 
AQ-U3128
Alias: 
2019-nCoV (S) ELISA Kit, Spike glycoprotein ELISA Kit, S glycoprotein ELISA Kit, E2 ELISA Kit, Peplomer protein ELISA Kit, Spike protein S1 ELISA Kit, Spike protein S2 ELISA Kit, Spike protein S2' ELISA Kit, S ELISA Kit, 2 ELISA Kit
Species: 
Coronavirus (COVID-19, SARS-CoV-2, 2019-nCoV)
Range: 
0.78-50pg/ml
Sensitivity: 
390fg/ml
Manual MSDS
  • SPECIFICATIONS
Product Name
COVID-19 Spike Protein(RBD) Accquant ELISA Kit
Alias
2019-nCoV (S) ELISA Kit, Spike glycoprotein ELISA Kit, S glycoprotein ELISA Kit, E2 ELISA Kit, Peplomer protein ELISA Kit, Spike protein S1 ELISA Kit, Spike protein S2 ELISA Kit, Spike protein S2' ELISA Kit, S ELISA Kit, 2 ELISA Kit
Catalogue No.
AQ-U3128
Size
48T/96T
Species
Coronavirus (COVID-19, SARS-CoV-2, 2019-nCoV)
UniProt No.
P0DTC2
Sample Type
血清，血浆，细胞培养上清液，细胞裂解液或组织裂解液，其它生物液体样品
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
5小时
Range
0.78-50pg/ml
Sensitivity
390fg/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with 2019-nCoV (S) , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml) OD-1 OD-2 Average
0 0.158 0.166 0.161
0.781 0.206 0.216 0.21
1.562 0.233 0.245 0.238
3.125 0.314 0.33 0.32
6.25 0.46 0.483 0.469
12.5 0.719 0.756 0.734
25 1.21 1.272 1.235
50 2.124 2.232 2.167
AQ-U3128 Standard Curve Image
Recovery

Add a certain amount of 2019-nCoV (S) into the sample. Calculate the recovery by comparing the measured value with the expected amount of 2019-nCoV (S) in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 88-104 96
EDTA plasma(n=10) 92-100 95
Heparin plasma(n=10) 90-105 95
Linearity

Dilute the sample with a certain amount of 2019-nCoV (S) at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 92-100% 88-105% 90-98%
EDTA plasma(n=10) 86-99% 90-101% 87-96%
Heparin plasma(n=10) 84-100% 83-94% 82-94%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/ml) 1.61 6.31 25.17 1.55 6.52 24.61
Standard deviation 0.07 0.36 1.39 0.08 0.31 1.3
CV(%) 4.49 5.72 5.54 4.88 4.68 5.27
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
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