COVID-19 Spike Protein Accquant ELISA Kit

Category:
Catalogue No.: 
AQ-U0001
Alias: 
2019 nCoV(S) ELISA Kit
Species: 
Coronavirus(COVID-19, SARS-CoV-2, 2019-nCoV, Novel Coronavirus Spike Glycoprotein, RBD, SARS-CoV-2 S1)
Range: 
3.12-200pg/ml
Sensitivity: 
1.875pg/ml
Manual MSDS
  • SPECIFICATIONS
Product Name
COVID-19 Spike Protein Accquant ELISA Kit
Alias
2019 nCoV(S) ELISA Kit
Catalogue No.
AQ-U0001
Size
48T/96T
Species
Coronavirus(COVID-19, SARS-CoV-2, 2019-nCoV, Novel Coronavirus Spike Glycoprotein, RBD, SARS-CoV-2 S1)
UniProt No.
P0DTC2
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
5 hours
Range
3.12-200pg/ml
Sensitivity
1.875pg/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with 2019 nCoV(S) , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml) OD-1 OD-2 Average
0 0.083 0.085 0.084
3.125 0.184 0.19 0.187
6.25 0.321 0.331 0.326
12.5 0.401 0.413 0.407
25 0.806 0.83 0.818
50 1.228 1.264 1.246
100 1.967 2.025 1.996
200 2.604 2.68 2.642
AQ-U0001 Standard Curve Image
Recovery

Add a certain amount of 2019 nCoV(S) into the sample. Calculate the recovery by comparing the measured value with the expected amount of 2019 nCoV(S) in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 89-102 95
EDTA plasma(n=10) 87-104 96
Heparin plasma(n=10) 86-101 93
Linearity

Dilute the sample with a certain amount of 2019 nCoV(S) at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 90-103% 88-101% 85-105%
EDTA plasma(n=10) 88-99% 84-99% 83-100%
Heparin plasma(n=10) 84-94% 80-96% 81-97%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/ml) 5.86 23.65 100.58 5.98 23.81 101.5
Standard deviation 0.32 1.11 6.34 0.28 1.36 6
CV(%) 5.54 4.68 6.3 4.68 5.72 5.91
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
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