anti- γ-H2AX antibody 
FNab10441
Catalogue No.
- Synonyms:
- gamma H2A.X, γH2AX, Phospho-H2AX-S139
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Isotype:
- IgG
- Tested Application:
- WB, IHC, IF
Product Information
- Product Name
- anti- γ-H2AX antibody
- Catalogue No.
- FNab10441
- Size
- 100µg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Host
- Rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background
- H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.
Immunogen Information
- Immunogen
- A synthetic phosphorylated peptide around S139 of human H2AX
- Synonyms
- gamma H2A.X, γH2AX, Phospho-H2AX-S139
- Observed MW
- 17 kDa
Application
- Tested Application
- WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:100
Uniprot information
- UniProt ID
- P16104
Images
- Image 1
-
Immunohistochemistry of paraffin-embedded mouse testis tissue slide using FNab10441(γ-H2AX Antibody) at dilution of 1:100
- Image 2
-
Immunofluorescent analysis of HeLa cells using FNab10441 (γ-H2AX antibody) at dilution of 1:100. HeLa cells were treated by UV for 15-30 minutes at RT.
- Image 4
-
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab10441(γ-H2AX Antibody) at dilution of 1:1000. NIH/3T3 cells were treated by UV for 15-30 minutes.
