anti- Phospho-CHEK1(S280) antibody
FNab09816
Catalogue No.
- Synonyms:
- CHK1
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Isotype:
- IgG
- Tested Application:
- ELISA, WB, IHC, IF
Product Information
- Product Name
- anti- Phospho-CHEK1(S280) antibody
- Catalogue No.
- FNab09816
- Size
- 100µg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Host
- Rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background
- The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Immunogen Information
- Immunogen
- Phospho-CHEK1-S280
- Synonyms
- CHK1
- Observed MW
- 60 kDa
Application
- Tested Application
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Uniprot information
- UniProt ID
- O14757
Images
- Image 2
-
Immunofluorescence analysis of MCF-7 cells using FNab09816. Blue: DAPI for nuclear staining
- Image 4
-
HT-29 cells were subjected to SDS PAGE followed by western blot with FNab09816 at dilution of 1:1000