Products
Vinculin antibody
- Product Name
- Vinculin antibody
- Catalogue No.
- FNab09800
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 5E7
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- vinculin
- Alternative Names
- CMD1W antibody, Metavinculin antibody, MVCL antibody, VCL antibody, Vinculin antibody
- UniProt ID
- P18206
- Observed MW
- 124 kDa
Application
- Tested Applications
- ELISA, FC, IF, IHC, WB
- Recommended dilution
- WB: 1:2000-1:20000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
hela lysates were subjected to SDS PAGE followed by western blot with FNab09800(Vinculin Antibody) at dilution of 1:5000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab09800(Vinculin Antibody) at dilution of 1:200
Immunofluorescent analysis of ( 4% PFA) fixed Hela cells using FNab09800 (vinculin antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Mouse IgG(H+L)
- Background
- Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament(F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin(124 kDa), is muscle-specific.
