Products

ZRANB3 antibody

Synonyms:DNA annealing helicase and endonuclease ZRANB3 antibody, Annealing helicase 2 antibody, AH2 antibody
Catalogue No.:FNab09756Reativity:Human
Host:RabbitTested Application:ELISA, WB
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
ZRANB3 antibody
Catalogue No.
FNab09756
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
zinc finger, RAN-binding domain containing 3
Alternative Names
DNA annealing helicase and endonuclease ZRANB3 antibody, Annealing helicase 2 antibody, AH2 antibody
UniProt ID
Q5FWF4
Observed MW
150 kDa and 50 kDa
Application
Tested Applications
ELISA, WB
Recommended dilution
WB: 1:200-1:2000; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09756(ZRANB3 Antibody) at dilution of 1:600
Background
DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.