Products
ZC3HAV1 antibody
- Product Name
- ZC3HAV1 antibody
- Catalogue No.
- FNab09606
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- zinc finger CCCH-type, antiviral 1
- Alternative Names
- DKFZp686F2052 antibody, DKFZp686H1869 antibody, DKFZp686O19171 antibody, FLB6421 antibody, FLJ13288 antibody, ZAP antibody, ZC3H2 antibody, ZC3HAV1 antibody, ZC3HDC2 antibody, Zinc finger antiviral protein antibody
- UniProt ID
- Q7Z2W4
- Observed MW
- 101 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:1000-1:10000; IHC: 1:20-1:200; IP: 1:1000-1:4000
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09606(ZC3HAV1 Antibody) at dilution of 1:4000
IP Result of anti-ZC3HAV1 (IP: FNab09606, 4ug; Detection: FNab09606 1:2000) with HeLa cells lysate 1000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab09606(ZC3HAV1 Antibody) at dilution of 1:50
- Background
- Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element(ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72(DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1(HIV-1), moloney and murine leukemia virus(MoMLV) and xenotropic MuLV-related virus(XMRV), filoviridae: ebola virus(EBOV) and marburg virus(MARV), togaviridae: sindbis virus(SINV) and Ross river virus(RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of DDX58/RIG-I signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes(ISGs).
