Products
YTHDF2 antibody
- Product Name
- YTHDF2 antibody
- Catalogue No.
- FNab09573
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- YTH domain family, member 2
- Alternative Names
- CLL associated antigen KW 14 antibody, HGRG8 antibody, NY REN 2 antibody, YTH domain family protein 2 antibody, YTH domain family antibody, member 2 antibody, YTHDF2 antibody
- UniProt ID
- Q9Y5A9
- Observed MW
- 68 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC, IP
- Recommended dilution
- WB: 1:1000-1:10000; IF: 1:10-1:100; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09573(YTHDF2 Antibody) at dilution of 1:5000
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab09573(YTHDF2 Antibody) at dilution of 1:50
- Background
- Specifically recognizes and binds N6-methyladenosine(m6A)-containing RNAs, and regulates mRNA stability(PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability(PubMed:22575960, PubMed:24284625, PubMed:25412658, PubMed:25412661). Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies(P-bodies), leading to mRNA degradation(PubMed:24284625, PubMed:26046440). Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation(PubMed:26458103).
